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KMID : 1098420040120030255
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Korean Journal of Medicinal Crop Science
2004 Volume.12 No. 3 p.255 ~ p.261
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A Cytotoxic Compound from Moutan Cortex Radicis
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Chu Bo-Yeun
Jin Wen-Yi
Choi Su-La
Myung Pyung-Keun
Seong Yeon-Hee
Song Kyung-Sik
Bae Ki-Hwan
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Abstract
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The MeOH extracts from 21 species were tested for their cytotoxicity against SK-MEL-28 melanoma cell line and HaCat normal cell line in 5 g/ml by sulforrhodamine-B (SRB) method. Among them, the MeOH extract from Moutan Cortex Radicis showed the moderate activity with the growth rate of 74.3% in SK-MEL-28 cells and the high activity with the growth rate of 207.8% in HaCat cells. Activity-guided fractionation was performed and five compounds, paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy- acetophenone (4), paeonfliorin (5) were isolated from hexane and EtOAc fraction. The structures were established by physicochemical and spectrometric methods (mp,;UV,;IR,;^1H-NMR,;^{13}C-NMR,;spectram). All compounds were evaluated for their cytotoxicity, compound 4 showed the significant cytotoxic activity with ED_50 value of 5.92;{mu}g/ml, but the other compounds no activity. These results suggest that compound 4 is a novel anticancer candidate against SK-MEL-28 melanoma cells.
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KEYWORD
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Moutan Cortex Radicis, cytotoxic compound, cytotoxicity, SK-MEL-28 melanoma cell, HaCat normal cell, sulforrhodamine-B
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